The human virome in health and disease - presented by Prof. Frederic Bushman

The human virome in health and disease

Prof. Frederic Bushman

Prof. Frederic Bushman
Slide at 18:42
Redondoviridae: a Novel Viral Family Assembled two genomes from lung donors Isolated another 17 genomes from our samples; also two TATTATTAT genomes reported by Cui et al., 2017 Member of the CRESS virus group Encode Cp (capsid), Rep (replication initiation) and third protein of unknown function (ORF3) New family: Redondoviridae, Spanish for "round" ORF3 Two species based on Rep similarity (Brisavirus and Vientovirus) 3.0 kb Human-associated; respiratory and oral Probably eukaryotic cell viruses: no CRISPR spacer matches, no prokaryotic Shine-Delgarno sequences Followed up with analysis of 7,581 further metagenomic samples Abbas, Taylor et al., Cell Host and Microb studio.streamyard.com is sharing your screen Stop sharing Hide
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References
  • 1.
    A. A. Abbas et al. (2019) Redondoviridae, a Family of Small, Circular DNA Viruses of the Human Oro-Respiratory Tract Associated with Periodontitis and Critical Illness. Cell Host & Microbe
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Summary (AI generated)

A redondo virus genome was assembled from two genomes obtained from lung donors. These genomes were used as probes against other respiratory samples, resulting in the discovery of 17 genomes. Another group, Coy et al. in 2017, reported two of these genomes as members of the CRESS virus group. The viruses encode Cp (capsid), Rep (replication initiation) protein, and a third protein of unknown function (ORF3). They were named Redondoviridae, which means "round" in Spanish. The viruses appear to be strictly human-associated, found in respiratory and oral samples.

Further investigation revealed that these viruses were not represented among CRISPR spacers, indicating they are not prokaryotic. Additionally, they do not have prokaryotic Shine-Dalgarno sequences, suggesting they may grow on human cells or eukaryotic organisms associated with humans. Analysis of over 7,581 metagenomic samples confirmed the presence of Redondoviruses, which were found to be associated with periodontitis and critical illness.

In periodontitis samples, redondo virus reads were observed and decreased with treatment in two separate studies.