The human virome in health and disease - presented by Prof. Frederic Bushman

The human virome in health and disease

Prof. Frederic Bushman

Prof. Frederic Bushman
Slide at 09:27
What is origin of the early life virome? Month 0 Day 0-4 Month 1 Month4 Month 1 (M0) (M1) (M4) n=20 Compare meconium/first bowel movement, one month, and four months in Month 4 healthy infants (n=20) Methods Purify virus-like particles (VLPs), count particles; shotgun sequence analysis Purify total stool DNA, shotgun analysis, targeted qPCR Isolate bacterial strains from early life samples, sequence genomes, test Epifluorescent image of VLPs phage production and replication in experimental infections Other methods studio.streamyard.com is sharing your screen Stop sharing Hide (Liang et al., Nature, 2020)
1
References
  • 1.
    G. Liang et al. (2020) The stepwise assembly of the neonatal virome is modulated by breastfeeding. Nature
Share slide
Summary (AI generated)

The origin of the early life virome was investigated through a series of experiments. Virus-like particles were purified and counted, then sequenced. Total stool DNA was also purified and analyzed using shotgun analysis and targeted qPCR. Bacterial strains from early life samples were isolated, their genomes sequenced, and phage production and replication were tested. The results of these studies will be summarized.