Introducing Transformative Plant Biotechnology: Multi-Knock – a multi-targeted transportome-scale amiRNA and CRISPR toolbox to overcome functional redundancy in plants - presented by Moran Anfang

Introducing Transformative Plant Biotechnology: Multi-Knock – a multi-targeted transportome-scale amiRNA and CRISPR toolbox to overcome functional redundancy in plants

Moran Anfang

Moran Anfang
Slide at 09:14
mTACT: Cell type-specific transportome-scale amiRNA
toolbox to overcome functional redundancy
ABCB10
Targets 2-8
closely
related
ABCB13
ABCB2
ABCB14
genes
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Summary (AI generated)

We have constructed 12 different cloning vectors to drive an amiRNA library and sublibraries. For example, I am interested in studying the plant hormone ABA and how it reaches the guard cells. I plan to use the KST1 promoter, which is specifically expressed in guard cells, to clone the library and screen for ABA-related phenotypes. This is just one specific example from the library to demonstrate a proof of concept.