Maintaining real-time operations poses significant challenges, which is why we focus on embedded systems. Setting up closed-loop experiments in this multidisciplinary field can be complex. To clarify, let's discuss the meaning of bio breed and the schematics for its setup. On the left side, you will find the biological preparation, where you can work at various levels such as in vitro or in vivo. In the in vitro setting, different types of neurons, like neuron cultures, can be studied.